Kourosh Azizi; Hedayat Dorzaban; Aboozar Soltani; Hamzeh Alipour; Seyed Aghil Jaberhashemi; Mostafa Salehi-Vaziri; Tahereh Mohammadi; Zahra Fereydouni; Azim 0000-0002-4167-5843 Paksa
Abstract
Background: Aedes mosquitoes transmit important arboviral diseases such as dengue to humans. This study was conducted to determine dengue virus infection in Aedes mosquitoes, emphasizing Aedes aegypti by Reverse Transcriptase-Polymerase Chain Reaction Assay from different regions in Southern Iran.Methods: ...
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Background: Aedes mosquitoes transmit important arboviral diseases such as dengue to humans. This study was conducted to determine dengue virus infection in Aedes mosquitoes, emphasizing Aedes aegypti by Reverse Transcriptase-Polymerase Chain Reaction Assay from different regions in Southern Iran.Methods: Aedes samples were collected by standard methods from different habitats of Hormozgan province, Southern Iran, in 2016-2017, and identified by morphological characteristics. In this study, TissueLyserII was used to homogenize the collected mosquitoes. In addition, the RT-PCR technique was used to identify dengue virus RNA.Results: Overall, 1351 larval and adult Aedes mosquitoes were collected from five sites in Hormozgan Province, including 452 adults and 899 larvae. Five species from Aedes genera were collected (Ae. aegypti, Ae. vittatus, Ae. caballus, Ae. caspius, Ae. vexans). The investigations of dengue virus infection in Aedes mosquitoes showed no dengue virus infection in this species.Conclusion: This study provides important information about Aedes mosquitoes. Vector control strategies must be emphasized and prioritized. Such actions prevent the establishment of Aedes mosquitoes and the spread of arboviral diseases in new areas. In addition, early detection of arboviruses in vectors and entomological monitoring can enhance the control measures for arbovirus diseases.
Jalal Mohammadi; Mohsen Kalantari; Zahra Nasiri; Davood Mehrabani; Hamzeh Alipour; Mohammad Djafar Moemenbellah-Fard; Kourosh Azizi
Abstract
Background: Visceral leishmaniasis (VL) is still widespread in tropical and subtropical regions worldwide. The vector of the disease is a sandfly, when infected with Leishmania parasite, can transmit the parasite from humans to other vertebrates. As knowledge about causative agents, vectors, and reservoirs ...
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Background: Visceral leishmaniasis (VL) is still widespread in tropical and subtropical regions worldwide. The vector of the disease is a sandfly, when infected with Leishmania parasite, can transmit the parasite from humans to other vertebrates. As knowledge about causative agents, vectors, and reservoirs of VL in Iran is necessary to control the disease, the present review study has focused on the disease elimination status from a health perspective in the country. Methods: In a recent review, various databases, such as Web of Science, PubMed, Scopus, Cochrane Library, Science Direct, Google Scholar, MEDLIB, Irandoc, Magiran, and SID were searched using the keywords “visceral leishmaniasis”, “VL”, “kala-azar”, “causative agent”, “parasite”, “vector”, “reservoir”, and “Iran”, and the retrieved data were extracted and reviewed independently during 2001-2019. Results: 475 published studies were recorded about VL in Iran from 2001 to 2021. 68 articles were later excluded from the study due to duplication after the initial review. Leishmania infantum and L. tropica were the causative agents, and dogs and humans were the reservoirs. The most common vectors were Phlebotomus major, P. kandelakii, P. keshishiani, P. alexandri, P. perfiliewi, and P. tobbi in different country regions. Conclusion: The prospect of eliminating VL in Iran is inadequate. Based on the findings, L. infantum and L. tropica were the causative agents, and humans and the Caninae subfamily were the VL reservoirs in the country. These findings can be added to the literature when health policymakers target preventive measures for VL in Iran.
Seyed Mohammad Amin Mahdian; Abbasali Raz; Navid Dinparast Djadid; Hamzeh Alipour
Abstract
Background: A given amino acid sequence can be encoded by a huge number of different nucleic acid sequences. These sequences, however, have proved not to be equally useful. The choice of sequence can significantly impact the expression of an encoded protein. Given the importance of protein-coding sequence ...
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Background: A given amino acid sequence can be encoded by a huge number of different nucleic acid sequences. These sequences, however, have proved not to be equally useful. The choice of sequence can significantly impact the expression of an encoded protein. Given the importance of protein-coding sequence and promising industrial and medicinal applications of Clostridium histolyticum collagenase, this study examined the codon optimization of the Col H gene so as to enhance collagenase expression in Escherichia coli (E. coli).
Methods: This is an experimental study in which the CDS of Col H gene was optimized according to the codon usage of E. coli, using Gene Designer software (DNA 2.0).
Results: The results revealed that relative frequency of codon usage in Col H gene was adapted to the most preferred triplets in E. coli in such a way that codon usage bias in E. coli was enhanced after codon optimization. The higher level of collagenase expression was more likely the result of substituting rare codons with optimal codons.
Conclusion: The findings of this study suggest that codon optimization provides a theoretical improvement in Col H gene expression in E. coli. Nevertheless, experimental research is needed to confirm the improvement.
Saeedeh Ebrahimi; Marzieh Shahriari-Namadi; Saeed Shahabi; Mohammad Djaefar Moemenbellah-Fard; Hamzeh Alipour
Abstract
Background:Human head lice is one of the most invincible neglected skin diseases. The use of pyrethroid insecticides is a standard method of treating the disease, which leads to lice population resistance in the long run. The main aim of the current survey was to screen the biomarkers of permethrin-associated ...
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Background:Human head lice is one of the most invincible neglected skin diseases. The use of pyrethroid insecticides is a standard method of treating the disease, which leads to lice population resistance in the long run. The main aim of the current survey was to screen the biomarkers of permethrin-associated kdr (knockdown resistance) point mutations through molecular analysis of the human head lice populations in primary school children in the south of Iran. Methods: In an experimental study, Field-collected head lice from infested students were fixed in ethanol, identified using valid taxonomic keys, and processed by PCR for kdr mutant studies. Sequencing partial voltage-sensitive sodium channel gene in different head lice populations was subsequently implemented and compared with the permethrin-resistant diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) as the gold standard. Results:Human head lice appeared to reflect kdr point mutations in specimens from the city of Shiraz. At least three amino acid mutations at designated sites of D820E, L840F, and N874G, corresponding to replacements of aspartic acid to glutamic acid, leucine to phenylalanine, and asparagine to glycine, are clear in this representative population, respectively. At the same time, only L840F is reported as a new mutant in this survey. Conclusion: The ongoing treatment of head lice infested in school children harboring kdr-mutated or permethrin-resistant mutants in Shiraz is risky, illogical, and contrary to the One Health initiative of the World Health Organization. Health executives should thus immediately take the indispensable steps to prohibit further procurement of permethrin.
Masoumeh Bagheri; Hamzeh Alipour; Ali Keshawarz
Abstract
Background: Scorpion sting is one of the serious and major medical problems in the world. Shiraz is a tourist city and one of the high-risk areas where a number of scorpion sting occurs annually.
Objectives: The present research aimed to conduct an epidemiological study of scorpion sting in Shiraz County.
Methods: ...
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Background: Scorpion sting is one of the serious and major medical problems in the world. Shiraz is a tourist city and one of the high-risk areas where a number of scorpion sting occurs annually.
Objectives: The present research aimed to conduct an epidemiological study of scorpion sting in Shiraz County.
Methods: The is a cross-sectional study performed using a checklists to collect data about scorpionism in Shiraz health center during 2014-2018.
Results: The results of the study showed that the total number of scorpion stings was 844 cases. generally, 336 cases (39.8%) were female and 508 s (59.3%) male. The results from the residential area revealed that 817 cases (96.8%) were urban and 27 (3.1%) were rural. The highest frequency of scorpionism happened in the 25-34 years old group (286,33.8%) and after that 15-24 year old (165,19.4%), 35-44 year old (157, 18.6%), 45-54 year old (108, 12.7%) groups, and the least frequency awas seen in the 0-4 year old group. The time periods between the sting and injection of anti-venom were less than 6 hours for 822, (85.5%) of cases, 6–12 hours for 101, (11.9%) of cases and, more than 12 hours for 21 (2.4%) cases. A total of 844 cases were recovered; 690 (81.7%) of them recovered with anti-venom and 154 (18.2%) injured individuals recovered without any anti-venom, the sting site was 27.6% in the foot, 57% in the hand, and 20% in the head and trunk.
Conclusion: Shiraz is a major tourist city, and most cases of scorpion-sting occur in summer and spring, when the tourist population is high, so health care providers must carry out an integrated program in those months.
Leila Izadpanah; Hamzeh Alipoor; Marzieh Shahriari-Namadi; Kourosh Azizi; Mohsen Kalantari; Abouzar Soltani; Masoumeh Bagheri
Abstract
Background: Sandflies are the vectors of at least eight different diseases, the most important of which is cutaneous leishmaniasis (CL). CL is a major public health problem in Iran, with annual cases increasing to more than 20,000 in 2019. Fars Province has the second-highest number of cases with more ...
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Background: Sandflies are the vectors of at least eight different diseases, the most important of which is cutaneous leishmaniasis (CL). CL is a major public health problem in Iran, with annual cases increasing to more than 20,000 in 2019. Fars Province has the second-highest number of cases with more than 3000 cases in 2019 in Iran. This study aimed to survey the fauna and different species of sandflies (Diptera: Psychodidae: Phlebotominae) in Fars Province, southwest Iran. Methods: This is a cross-sectional study. Sandflies were collected in urban and rural regions using the sticky-traps method, and then they were cleared and fixed in puris medium and identified using the pictorial key. Results: A total of 1071 of sandflies were collected. The dominant species consisted of 5 species of Phlebotomus (Ph. papatasi 55.4%, Ph. alexandri 17.4%, Ph.sergenti 5.6%, Ph. caucasicus 1.4%, Ph. ansari 0.46% and 6 species of Sergentomyia (Ser. sintoni 10.8%, Ser. antennata 5.4%, Ser. tiberiadis 1.4%, Ser. tobbi 1%, Ser. baghdadis 0.5%, and Ser. halepensis 0.09%. Conclusion: This investigation showed that Ph. papatasi was the most prevalent species playing a crucial role in the transmission of cutaneous leishmaniasis in Fars Province. Species of Ph. papatasi and Ph. sergenti are the main vectors of CL in Iran. The healthcare system must take steps to control cutaneous leishmaniasis, raise awareness of the disease, and apply effective ways to prevent it.
Mohammad Reza Fakoorziba; Mohammad Djaefar Moemenbellah-Fard; Kourosh Azizi; Heshmatollah Shekarpoor; Hamzeh Alipoor
Volume 2, Issue 1 , January 2014, , Pages 2-7
Abstract
Background: Medicinal plant extracts such as those obtained from Salvia species have a wide variety of chemical compounds in their essential oils. The repellency of a number of essential oils including those from the labiates like Salvia against several insect species including the common house flies ...
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Background: Medicinal plant extracts such as those obtained from Salvia species have a wide variety of chemical compounds in their essential oils. The repellency of a number of essential oils including those from the labiates like Salvia against several insect species including the common house flies is reported. Objective: The aim of this investigation was to find out the excito-repellency effects of Salvia sclarea L. (Lamiaceae) extracts against adult house flies, Musca domestica L. (Diptera: Muscidae). Methods: Air-dried plant material from the aerial parts of S. sclarea was subjected to hydro-distillation in a Clevenger type glass apparatus model Soxhlet with acetone, benzene, petroleum ether, chloroform, and aqueous solvents. Only adult house flies were inserted into an exposure chamber and their behavior was monitored for feeding tendency, repellency rate and deterrence rate. Statistical analyses were carried out by one-way analysis of variance (ANOVA) with computation of the significance of differences in the outcome of various treatments. Results: There were significant differences among most of the various extracts with their controls in the sequential effects of feeding (P=0.04), deterrent (P=0.023) and repellency (P=0.01) rates of house flies. The order of potency for various extracts with a concentration of 100 g/l was as follows: petroleum ether> benzene> water> acetone> chloroform. Conclusion: It is thus conceivable to search for native means of combating house flies by fractionating the active ingredients in the Clary sage in the light of its excito-repellency effects.
Hamzeh Alipour; Mohammad Reza Abaie; Hossein Ladonni; Ali Akbar Kadivar
Volume 1, Issue 2 , October 2013, , Pages 94-97
Abstract
Background: Malaria is the most important vector-borne disease in many tropical countries all over the world. Because of the widespread use of pyrethroid insecticide treated mosquito nets in the world, the effects of excito-repellency (ER) phenomenon of pyrethroids against main malaria vector, Anopheles ...
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Background: Malaria is the most important vector-borne disease in many tropical countries all over the world. Because of the widespread use of pyrethroid insecticide treated mosquito nets in the world, the effects of excito-repellency (ER) phenomenon of pyrethroids against main malaria vector, Anopheles stephensi were studied.Methods: The ER phenomenon of three concentrations of two synthetic pyrethroids (permethrin and deltamethrin) as well as etofenprox was evaluated against Anopheles stephensi under laboratory conditions. Female 5-7 day unfed mosquitoes were exposed to animal bait in holder and the animal’s back and head were covered with impregnated bed net in -ER test chamber.Results: Deltamethrin was more effective compared to other insecticides in killing the mosquitoes. The mean of entry to exit trap showed significant differences in all concentrations of insecticides (P<0.05).Conclusion: This study showed that ER phenomenon of insecticides should be noticed in vector control programs. The ranked data indicated the relative potency of both pyrethroids and etofenprox. Deltamethrin repels the female mosquitoes more than other insecticides tested.