Jalal Mohammadi; Mohsen Kalantari; Zahra Nasiri; Davood Mehrabani; Hamzeh Alipour; Mohammad Djafar Moemenbellah-Fard; Kourosh Azizi
Abstract
Background: Visceral leishmaniasis (VL) is still widespread in tropical and subtropical regions worldwide. The vector of the disease is a sandfly, when infected with Leishmania parasite, can transmit the parasite from humans to other vertebrates. As knowledge about causative agents, vectors, and reservoirs ...
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Background: Visceral leishmaniasis (VL) is still widespread in tropical and subtropical regions worldwide. The vector of the disease is a sandfly, when infected with Leishmania parasite, can transmit the parasite from humans to other vertebrates. As knowledge about causative agents, vectors, and reservoirs of VL in Iran is necessary to control the disease, the present review study has focused on the disease elimination status from a health perspective in the country. Methods: In a recent review, various databases, such as Web of Science, PubMed, Scopus, Cochrane Library, Science Direct, Google Scholar, MEDLIB, Irandoc, Magiran, and SID were searched using the keywords “visceral leishmaniasis”, “VL”, “kala-azar”, “causative agent”, “parasite”, “vector”, “reservoir”, and “Iran”, and the retrieved data were extracted and reviewed independently during 2001-2019. Results: 475 published studies were recorded about VL in Iran from 2001 to 2021. 68 articles were later excluded from the study due to duplication after the initial review. Leishmania infantum and L. tropica were the causative agents, and dogs and humans were the reservoirs. The most common vectors were Phlebotomus major, P. kandelakii, P. keshishiani, P. alexandri, P. perfiliewi, and P. tobbi in different country regions. Conclusion: The prospect of eliminating VL in Iran is inadequate. Based on the findings, L. infantum and L. tropica were the causative agents, and humans and the Caninae subfamily were the VL reservoirs in the country. These findings can be added to the literature when health policymakers target preventive measures for VL in Iran.
Saeedeh Ebrahimi; Marzieh Shahriari-Namadi; Saeed Shahabi; Mohammad Djaefar Moemenbellah-Fard; Hamzeh Alipour
Abstract
Background:Human head lice is one of the most invincible neglected skin diseases. The use of pyrethroid insecticides is a standard method of treating the disease, which leads to lice population resistance in the long run. The main aim of the current survey was to screen the biomarkers of permethrin-associated ...
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Background:Human head lice is one of the most invincible neglected skin diseases. The use of pyrethroid insecticides is a standard method of treating the disease, which leads to lice population resistance in the long run. The main aim of the current survey was to screen the biomarkers of permethrin-associated kdr (knockdown resistance) point mutations through molecular analysis of the human head lice populations in primary school children in the south of Iran. Methods: In an experimental study, Field-collected head lice from infested students were fixed in ethanol, identified using valid taxonomic keys, and processed by PCR for kdr mutant studies. Sequencing partial voltage-sensitive sodium channel gene in different head lice populations was subsequently implemented and compared with the permethrin-resistant diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) as the gold standard. Results:Human head lice appeared to reflect kdr point mutations in specimens from the city of Shiraz. At least three amino acid mutations at designated sites of D820E, L840F, and N874G, corresponding to replacements of aspartic acid to glutamic acid, leucine to phenylalanine, and asparagine to glycine, are clear in this representative population, respectively. At the same time, only L840F is reported as a new mutant in this survey. Conclusion: The ongoing treatment of head lice infested in school children harboring kdr-mutated or permethrin-resistant mutants in Shiraz is risky, illogical, and contrary to the One Health initiative of the World Health Organization. Health executives should thus immediately take the indispensable steps to prohibit further procurement of permethrin.
Zahra Hosseini; Mostafa Salehi Vaziri; Sara Ahmadnia; Mohammad Reza Fakoorziba; Tahmineh Jalali; Zakkyeh Telmadarraiy; Mohammad Djaefar Moemenbellah-Fard
Abstract
Background: Crimean-Congo hemorrhagic fever (CCHF) is a neglected tick-borne viral zoonotic disease. The aim was to detect CCHF virus (CCHFV) among wild ticks from Artiodactyla, Bos taurus, Ovis aries, and Capra hircus, in a previously declared CCHFV-free province of Kohgiluyeh Boyer-Ahmad, southwest ...
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Background: Crimean-Congo hemorrhagic fever (CCHF) is a neglected tick-borne viral zoonotic disease. The aim was to detect CCHF virus (CCHFV) among wild ticks from Artiodactyla, Bos taurus, Ovis aries, and Capra hircus, in a previously declared CCHFV-free province of Kohgiluyeh Boyer-Ahmad, southwest Iran. Methods: From April to November 2015, hard ticks were collected in a cross-sectional study and checked by microscope for species identity from ungulates in 51 study villages. About 55% of the ticks were then subjected to reverse-transcription polymerase chain reaction (RT-PCR) to detect CCHFV genome. Results: Overall, 859 hard ticks were captured, from which 8 different species in two genera were identified. The genus Rhipicephalus was distributed in half (#26) of the study villages. It was the most frequent (≈60%) tick genus. Hyalomma anatolicum, H. asiaticum, H. excavatum, H. marginatum, H. scupense, Rhipicephalus sanguineus, R. turanicus, and R. bursa were identified on the ruminants. From 469 adult ticks subjected to RT-PCR, one (0.2%) tick, R. bursa, was positive with CCHFV genome. It was from a cold hardy highland village in Dena County. It had CCHFV RNA for the first time from this region. Conclusion: The detection of CCHF viral RNA in one hard tick species, R. bursa, was confirmed in the southwest of Iran, thus partially indicating CCHFV presence of ticks in this region.
Kourosh Azizi; Aboozar Soltani; Shokat Ali Amiri; Mohammad Reza Fakoor Ziba; Mohammad Djaefar Moemenbellah-Fard
Abstract
Background: The application of insecticides against vector mosquito larvae is a crucial step to control human malaria. Insecticide resistance is a major impediment to vector control strategies. The main aim of this study was to conduct laboratory and semi-field evaluations on lethal and residual effects ...
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Background: The application of insecticides against vector mosquito larvae is a crucial step to control human malaria. Insecticide resistance is a major impediment to vector control strategies. The main aim of this study was to conduct laboratory and semi-field evaluations on lethal and residual effects of temephos and pyriproxyfen insecticides against malaria mosquito larvae, Anopheles stephensi. Methods: Both susceptibility test and residual bioassay were performed to assess the lethal concentrations of each insecticide on 50% (LC50) of the IV instars larval populations and their activity periods according to standard protocols of WHO. Nine and eleven different concentrations with two sets of control in each case were applied for temephos and pyriproxyfen, respectively. Data were analyzed using probit analysis and SPSS software. Results: The LC50 and LC90 for temephos and pyriproxyfen under laboratory conditions were 0.4 and 0.63, and 1.69 × 10-4 and 4.036 × 10-4 ppm, respectively. Although the field strain of An. stephensi larvae was completely susceptible to pyriproxyfen, there was noticeable resistance (8% mortality at the diagnostic dose) to temephos in Nikshahr County, Southeast Iran. This is the first report of resistance to temephos for this malaria main vector in Iran. Depending on the applied variable doses, the residual effects of temephos and pyriproxyfen under semi-field conditions lasted maximally for 3 and 10 weeks, respectively. Conclusion: The high lethal and residual effects of pyriproxyfen on mosquito larvae confer an unprecedented opportunity in vector control operations leading to elimination of malaria in Iran.
Seyed Aghil Jaberhashemi; Mohammad Djaefar Moemenbellah-Fard; Mousa Khosravani; Mojtaba Norouzi; Hadi Kalari
Abstract
Objective: The main aim of this research is to investigate the knowledge, attitude and practice (KAP) on malaria control with respect to indoor residual spraying (IRS) in a community of Southern Iran. Introduction: Human malaria is an important vector-borne infectious disease in Iran. It remains endemic ...
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Objective: The main aim of this research is to investigate the knowledge, attitude and practice (KAP) on malaria control with respect to indoor residual spraying (IRS) in a community of Southern Iran. Introduction: Human malaria is an important vector-borne infectious disease in Iran. It remains endemic over most parts of the oriental region of Iran that is still in the pre-elimination phase without considerable drop in the proportion of malaria cases. Methods: This research was a cross-sectional household survey conducted in Kazerun, Fars province, Iran, to examine he malaria status and identify the parameters that obstruct the progress of the elimination phase. A standard questionnaire with 20 questions was provided to households including knowledge, attitude and practice categories. Sampling was then stratified to choose the samples in three rural foci of Kazerun. Results: The sex ratio of participants included 192 females (92.3%) and 16 males (7.7%), respectively. This community benefited mostly from acceptable literacy level. A strong association prevailed between the majority of respondents’ (88.5%) knowledge of fever and chills as the main symptoms of malaria and their literacy level. The majority (198, 95.2%) of volunteers declared that malaria disease was transmitted through the mosquito bites. In line with this study, literate persons have raised motivation and attitude to use bednets and keep themselves away from mosquitoes. These individuals cooperate perfectly with healthcare workers in malaria control program strategies (especially IRS). Conclusion: Comprehensive and synergistic measures are needed to be taken to manage malaria elimination strategy in Iran.
Ehsan Saki; Abouzar Soltani; Mohammad Djaefar Moemenbellah-Fard; Mohsen Kalantari; Hedayat Dorzaban; Kourosh Azizi
Volume 6, Issue 1 , January 2018, , Pages 2-7
Abstract
Background: Fipronil systemic insecticide and integrated vector management (IVM) leading to control and/or reduction of zoonotic cutaneous leishmaniasis (ZCL) cases were evaluated in the studied endemic foci of Fars province, southern Iran, during 2016 to 2017. Methods: Based on available data on disease ...
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Background: Fipronil systemic insecticide and integrated vector management (IVM) leading to control and/or reduction of zoonotic cutaneous leishmaniasis (ZCL) cases were evaluated in the studied endemic foci of Fars province, southern Iran, during 2016 to 2017. Methods: Based on available data on disease circumstances collected from Center for Disease Control (CDC) of Shiraz University of Medical Sciences, incidence of disease, and demographics of the natives, five villages were randomly selected among those with high and medium CL infection in Kharameh area. All villagers were checked by physical examination and the incidence rates of ZCL cases were recorded. IVM interventions were implemented. Indeed, these procedures consisted of fipronil systemic insecticide poison baits, indoor residual spraying (IRS), outdoor spraying of the patients’ houses, and the thermal fogging (three times) during peak periods of sand flies’ activities. Results: After interventions, the incidence rates of ZCL decreased by 2.55% in Mehrabad village, in which all IVM methods were implemented. Similarly, the incidence of disease was reduced by 4.89% in Sofla and Moezabad villages, using exclusively fipronil poison baits. Besides, these incidence rates declined by 1.15% in two control villages of Soltan-Shahr and Abshor, where examination of the rodent reservoir hosts was performed. Conclusion: Fipronil and IVM methods were advantageous in reducing the incidence rates of leishmaniasis, but it seems that the use of fipronil systemic insecticide as a poison bait against reservoir rodent was significantly more effective (P-Value=0.01).
Zahra Zamanian; Shahrzad Riaei; Najmeh Kaveh; Amineh Khosravani; hadi daneshmandi; Mehrab Sayadi; Mohammad Djaefar Moemenbellah-Fard
Volume 4, Issue 1 , January 2016, , Pages 7-13
Abstract
Background: Depression is one of the mental disorders which have become a public health problem throughout of the world. The objective of this study was to investigate depression and to determine its correlated factors among students at Shiraz University of Medical Sciences (SUMS).Methods: 358 students ...
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Background: Depression is one of the mental disorders which have become a public health problem throughout of the world. The objective of this study was to investigate depression and to determine its correlated factors among students at Shiraz University of Medical Sciences (SUMS).Methods: 358 students of SUMS participated in this crosssectional study in 2012. The participants were selected by Proportion Partition sampling method. A two-part questionnaire was used as the data collecting tool. In the first part, demographic characteristics and in the second part the Beck Depression Inventory (BDI) were included.Results: Mean score of depression in the studied population was 10.98. More than half of all the participants (54.7%) were symptomless; 41% of them were in the weak to moderate depression categories, and 4.2% in the strong and very strong categories. The data showed a significant relationship between scores of depression and marital status, academic grade, field content of study, use of psychoactive drugs, job outlook, problem with marriage and religious commitments. The depression scores in the married, religious and postgraduate participants and in those participants with course pleasure and excellent career perspective were lower than those in the other groups.Conclusion: Taking measures to reduce the factors leading to mental disorders is recommended. The involvement of students in socio-cultural, recreational and sport activities, reinforcement of consultation and clinical psychological services, and promotion of religious beliefs are instrumental in the enhancement of moral values and amelioration of depression among university students.
