Document Type: Original Articles

Authors

Department of Medical Entomology, Research Center for Health Sciences, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran.

Abstract

Background: Human malaria remains a major malady in Eastern Iran. Vector control interventions such as indoor residual spraying are used to fight with the disease. This study was undertaken to determine the lethal and residual effects of three different pyrethroid insecticides on adult mosquitoes of Anopheles stephensi on different surfaces in Iran, as part of a national program to monitor insecticide resistance in endemic areas. Methods: Two main endemic foci were selected as collection sites. Wild adult females of An. stephensi (mysoriensis strain) from the first focus were subjected to standard susceptibility tests, using lambdacyhalothrin, deltamethrin and cyfluthrin insecticides within holding tubes according to the method proposed by WHO. In Kazerun, the residual effects of these compounds were examined by conical bioassay tests of An. stephensi (type strain) on plaster and cement walls. Data were analyzed using Mann-Whitney test to determine the differences in susceptibility and residual effects of An. stephensi mosquitoes to these insecticides. Results: The susceptibility of females of An. stephensi to three concentrations of lambdacyhalothrin, deltamethrin and cyfluthrin insecticides culminated in full scale mortality at the highest diagnostic dose. The maximal residual time of these three insecticides on plaster and cement walls was estimated to be about three months. There was no significant difference in the mortalities of An. stephensi on different sprayed surfaces (P=0.653). Conclusion: All field-collected An. stephensi populations exhibit gross susceptibility to all diagnostic doses of the three evaluated insecticides. In endemic areas, lambdacyhalothrin reveals a slightly longer residual activity than the other two insecticides.

Keywords

  1. Moemenbellah-Fard MD, Saleh V, Banafshi O,
  2. Dabaghmanesh T. Malaria elimination trend from a
  3. hypo-endemic unstable active focus in southern Iran:
  4. predisposing climatic factors. Pathog Glob Health.
  5. ;106(6):358-65.
  6. Glick JI. Illustrated key to the female Anopheles of
  7. southwestern Asia and Egypt (Diptera: Culicidae).
  8. Mosq Systemat. 1992;24:125-53.
  9. Alipour H, Ladonni H, Abaie MR, Moemenbellah-
  10. Fard MD, Fakoorziba MR. Laboratory efficacy tests
  11. of pyrethroid-treated bed nets on the malaria vector
  12. mosquito, Anopheles stephensi, in a baited excitorepellency
  13. chamber. Pak J Biol Sci. 2006;9(10):1877-83.
  14. Moemenbellah-Fard MD (editor) Malaria vector control
  15. and personal protection: including indoor residual
  16. spraying. Global Malaria Program, World Health
  17. Organization. 2008; 110 p.
  18. Rafinejad J, Vatandoost H, Nikpoor F, Abai MR,
  19. Shaeghi M, Duchen S, et al. Effect of washing on the
  20. bio-efficacy of insecticide-treated nets (ITNs) and
  21. long-lasting insecticidal nets (LLINs) against main
  22. malaria vector Anopheles stephensi by three bioassay
  23. methods. J Vector Dis. 2008;45(2):143-50.
  24. Ruzo LO, Casida JE. Metabolism and toxicology of
  25. pyrethroids with dihalovinyl substituents. Environ
  26. Health Perspect. 1977;21:285-92.
  27. Raeisi A, Abai MR, Akbarzadeh K, Nateghpour M,
  28. Sartipi M, Hassanzehi A, et al. Residual effects of
  29. deltamethrin WG 25% as a new formulation on different
  30. surfaces against Anopheles stephensi, in south eastern
  31. Iran. J Arthropod Dis. 2010;4(1):60-5.
  32. WHO. Test procedure for insecticide resistance
  33. monitoring in malaria vectors, bio-efficacy and
  34. persistence of insecticides on treated surfaces. WHO/
  35. CDS/CPC/MAL/98.12. World Health Organization,
  36. Geneva, 1998.
  37. Soltani A, Vatandoost H, Oshaghi MA, Enayati AA,
  38. Raeisi A, Eshraghian MR, et al. Baseline susceptibility
  39. of different geographical strains of Anopheles stephensi
  40. (Diptera: Culicidae) to temephos in malarious areas of
  41. Iran. J Arthropod Dis. 2013;7(1):56-65.
  42. Hanafi-Bojd AA, Vatandoost H, Oshaghi MA,
  43. Haghdoost AA, Shahi M, Sedaghat MM, et al.
  44. Entomological and epidemiological attributes for
  45. malaria transmission and implementation of vector
  46. control in southern Iran. Acta Trop. 2012;121(2):85-92.
  47. Vatandoost H, Hanafi-Bojd AA. Indication of
  48. pyrethroid resistance in the main malaria vector
  49. Anopheles stephensi from Iran. Asian Pac J Trop Med.
  50. ;5(9):722-6.
  51. Moemenbellah-Fard MD, Benafshi O, Rafinejad J,
  52. Ashraf H. Tick-borne relapsing fever in a new highland
  53. endemic focus of western Iran. Ann Trop Med Parasitol.
  54. ;103(6):529-37.
  55. Fakoorziba MR, Baseri A, Eghbal F, Rezaee S, Azizi
  56. K, Moemenbellah-Fard MD. Post-earthquake outbreak
  57. of cutaneous leishmaniasis in a rural region of southern
  58. Iran. Ann Trop Med Parasitol. 2011;105(3):217-24.
  59. Fakoorziba MR, Eghbal F, Hassanzadeh J,
  60. Moemenbellah-Fard MD. Cockroaches (Periplaneta
  61. americana and Blattella germanica) as potential
  62. vectors of the pathogenic bacteria found in nosocomial
  63. infections. Ann Trop Med Parasitol. 2010;104(6):521-8.
  64. Azizi K, Fakoorziba MR, Jalali M, Moemenbellah-Fard
  65. MD. First molecular detection of Leishmania major
  66. within naturally infected Phlebotomus salehi from a
  67. zoonotic cutaneous leishmaniasis focus in southern
  68. Iran. Trop Biomed. 2012;29(1):1-8.
  69. Abtahi M, Shayeghi M, Khoobdel M, Vatandoost H,
  70. Abaei MR, Akbarzadeh K. Persistence and residue
  71. activity of deltamethrin on indoor residual spraying
  72. surfaces against malaria vectors in southeastern Iran.
  73. Asian Pac J Trop Biomed. 2011;S271-S275.
  74. Abbott WS. A method of comparing the effectiveness
  75. of an insecticide. J Econ Entomol. 1925;18:265-7.
  76. Azizi K, Soltani A, Poodat A, Khodadadi M, Yaran
  77. M, Hasanvand B. Susceptibility of Anopheles stephensi
  78. against five current chemical insecticides. J Hormozgan
  79. Univ Med Sci. 2010;14(4):305-11.
  80. Vatandoost H, Abai MR, Abbasi M, Shaeghi M,
  81. Abtahi M, Rafie F. Designing of a laboratory model
  82. for evaluation of the residual effects of deltamethrin
  83. (K-othrine WP 5%) on different surfaces against
  84. malaria vector, Anopheles stephensi (Diptera:
  85. Culicidae). J Vector Dis. 2009;46(4):261-7.
  86. Ladonni H, Motabar M, Iranpour M. Residual effect
  87. of lambdacyhalothrin (Icon 10% WP) on different
  88. surfaces in south of Iran. Iranian J Publ Health.
  89. ;23(1-4):21-32.