Document Type: Original Articles

Authors

1 Qazvin University of medical science, Qazvin, Iran

2 Children Growth and Development Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

3 Qazvin University of Medical Sciences, Qazvin, Iran;

4 Head of Department of Food Hygiene and Quality Control, School and Research Center of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Background: The Prangos ferulacea (PF)–yogurt is a traditional food in Iran. This study investigated the effects of PF on the microbial, physicochemical and sensory properties of probiotic yoghurt.Methods: Pasteurized low fat milk was heated up to 85°C, cooled to 40°C, and then mixed with conventional and Lactobacillus casei starter cultures incubated at 37°C until pH decreased to 4.6. Then, the cooked PF was added to yogurt and stored at 5°C for 21 days. Acidity, Syneresis, probiotic colony count and sensory evaluation of yoghurt was determined during the storage time. The experiments were replicated for three times. Probiotic yogurt (PY) was examined as the control and probiotic yoghurt containing 10, 20 and 30% Prangos ferulacea (PFY) as the samples.Results: Total titratable acidity of PFYs compared to PY was not significant during 21 days. The syneresis rate of PFY yogurt showed significant differences compared to PY during storage time (P<0.001). Comparison of the mean scores of sensory attributes (taste, odor, syneresis, mouth feel and color) of PFY yogurt showed that there were no significant differences with PY. Enumeration of lactobacillus casei (logCFU/mL) revealed significant differences in PFYs compared to PY in each experimental day (P=0.040). At the end of the storage time (day 21), the highest number of L.casei was observed in PY and PFY 20% and the lowest in PFY30%.Conclusion: Adding Prangos ferulacea (20%) to probiotic yogurt prevented an increase in acidity, a decrease in syneresis rate and an increase in the number of probiotic bacteria during 21 days. Results showed that the highest number of probiotic bacteria was seen in probiotic yogurt containing 20 percent Prangos ferulacea; probably, the existing fiber and some nutrients in Prangos ferulacea promoted the viability of probiotic bacteria.

Keywords

  1. Evans WC. Trease and Evans, Pharmacognosy, 13th ed. 1989; Bailliere Tindall, London.
  2. Mozaffarian VA, Dictionary of Iranian Plant Names. Farhang Moaser LTD. Tehran.1996; (in Persian).
  3. Zargari A. Medicinal Plants. 1988; Vol. 12, Tehran University Publications, Tehran (in Persian).
  4. Baser K, Ermin N, Adiguzel N, Aytac Z. Composition of the Essential Oil of Prangos ferulacea (L.) Lindl. JEOR 1996; 8: 297-8.
  5. Ulubelen A, Topcu G, Tan N, Olçal S, Johansson C, Uçer M, et al. Biological-Activities of a Turkish Medicinal Plant, Prangos-Platychlaena. J Ethnopharmaco 1995; 45: 193-7.
  6. Chapman & Hall, Dictionary of Natural P roducts, London. 1998; Vol. 7.
  7. Sajjadi SE, Shokoohinia Y, Gholamzadeh S. Res Chemical composition of essential oil of Prangos ferulacea (L.) Lindl. roots. Chemija 2011; 22, 178-80.
  8. Razavi SM. Chemical Composition and Some Allelopathic Aspects of Essential Oils of (Prangos ferulacea L.) Lindl at Different Stages of Growth. JAST 2012; 14: 349-56.
  9. Coskun B, Gulsen N, Umucalılar HD. The nutritive value of Prangos ferulacea. GFS 2004; 1(59): 15-9.
  10. Coruh N, Celep AGS, Ozgokce F. Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione- S-transferase. Food Chemistry 2007; 100: 1237-42.
  11. Massumi M, Fazeli M, Alavi SHR, Ajani Y. Chemical constituents and antibacterial activity of essential oil of Prangos ferulacea (L.) Lindl. Fruits. IJPhS 2007; 3: 171-6.
  12. Zacarchenco PB, Roig MS. Properties of Streptococcus thermophilus fermented milk containing variable concentration of Bifidobacterium longum and Lactobacillus acidophilus. BJM 2006; 37: 338-44.
  13. Salminen S, Gibson C, Bouley MC, Cummings JH, Franck A, Gibson GR, et al. Gastro intestinal physiology and function: the role of prebiotics and probiotics. BJN 1998; 80 (Suppl 1): S147–71.
  14. Homayouni Rad A. Functional Dairy Probiotic Food Development: Trends, Concepts, and Products. 2008; intech ltd. Chapter 9. [Internet]. Available from: http:// dx.doi.org/10.5772/48797
  15. Homayouni Rad A. Therapeutical effects of functional probiotic, prebiotic and synbiotic foods. 2008; Iran, Tabriz: Tabriz University of Medical Sciences, first edition (in Persian).
  16. Goldin BR, Gorbach SL. Clinical indications for probiotics: an overview. Clin Infect Dis 2008; 46 (Suppl 2): S96 100.
  17. Kaur IP, Kuhad A, Garg A, Chopra K. Probiotics: delineation of prophylactic and therapeutic benefits. JMF 2009; 12: 219-35.
  18. Iyer RN. Hittinahalli V. Modified Pap method to among methicillin resistant Staphylococcus aureus isolates tertiary care hospital. IJMM 2008; 26(2): 176-9.
  19. Matsuzaki T. Health properties of fermented milk with Lactobacillus casei strain shirota (LcS). Handbook of
  20. Fermented Functional Food, (ed. E. R. Farnworth), CRC press LLC. Boca Raton 2003; 145-75.
  21. Khan SH, Ansari FA. Probiotics – the friendly bacteria with market potential in global market. PJPhS 2007; 20(1): 71-6.
  22. Tharmaraj N, Shah NP. Selective Enumeration of Lactobacillus delbrueckii ssp. Bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacteria, Lactobacillus casei, Lactobacillus rhamnosus, and Propionibacteria. JDS 2003; 86: 2288-96.
  23. Iran standard organization. Milk and milk productsyoghurt- specifications and test methods Numbers 695 and 2852. Iran: Iran national standard; 2002 (in Persian).
  24. Mazloomi SM, Shekarforoush SS, Ebrahimnejad H, Sajedianfard J. Effect of adding inulin on microbial and physicochemical properties of low fat probiotic yogurt. IJVR [Internet]. Spring 2011; 2(12): 87-163. Available from: http://ijvr.shirazu.ac.ir
  25. Yeganehzad S, Mazaheri-Tehrani M, Shahidi F. Effect of soy bean milk on microbial, physicochemical and organoleptic properties of probiotic yogurt. 18th National Congress of Food Industry, University of Uromia, Iran, 2008; http://profdoc.um.ac.ir/paperabstract- 1010488.html (in Persian).
  26. Aghajani AR, Pourahmad R, Mahdavi Adeli HR. The effect of Oligofructose, Lactulose and Inulin Mixture as Prebiotic on Physicochemical Properties of Synbiotic Yogurt. JFBT 2014; 2(4) 33-40 (in Persian).
  27. Aryana KJ, McGrew P. Quality attributes of yogurt with Lactobacillus casei and various prebiotics. Swiss Society of Food Science and Technology. 2007; Published by Elsevier Ltd. LWT 40: 1808-14.
  28. Zainoldin KH, Baba AS. The Effect of Hylocereus polyrhizus and Hylocereus undatus on Physicochemical, Proteolysis, and Antioxidant Activity in Yogurt. Kuala lumpur, Malaysia, World Academy of Science, Engineering and Technology 2009; 60: 361-6.
  29. Dehghani N, Pour Ahmad R, Nemati N. Effect of inoculation Cell culture and incubation temperature on probiotic yogurt containing Lactobacillus casei. Food Technology & Nutrition 2013; 2(10).
  30. Sendra E, F ayos P, L ario Y, Fernandez-Lopez J, Sayas-Barbera E, P erez-Alvarez JA. Incorporation
  31. of citrus fibers in fermented milk containing probiotic bacteria. Food Microbiology 2008; 1(25):13-21.
  32. Donkor ON, Nilmini SLI, Stolic P, Vasiljevic T, Shah NP. Survival and activity of selected probiotic organisms in set-type yoghurt during cold storage. IDJ 2007; 6(17): 657-65.