Document Type : Original Articles

Authors

1 Department of Environmental Health Engineering, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran;

2 Research Center of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran;

3 Department of Medical Entomology, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Background: The consumption of healthy food is considered as an essential need to devoid the physical, chemical, and biological hazards. The importance of this issue is more conspicuous in places such as hospitals where people with somehow compromised immune systems are under treatment. Therefore, this research aimed to evaluate the microbiological quality of food contact surfaces in a kitchen in one of the hospitals of Shiraz University of Medical Sciences. Methods: In the present study, samples were taken from 48 food contact surfaces according to ISO 18593:2004(E) and placed into the bags containing diluting solution; they were then transferred to the laboratory for microbial analysis in the cold chain. The microbial analysis was carried out according to ISO 4833-1:2013 and BS ISO 4832:2006 for enumeration of total bacterial count and coliform. Results: Based on the results presented here, 39.6% and 85.7% of the samples showed acceptable contamination with regard to the enumeration of total bacterial and coliform count. Besides, 18.2% and 72.7 % of work surfaces groups (cutting board, table, and hand) showed acceptable contamination with regard to the enumeration of total bacterial count and coliform in comparison to the standards. Furthermore, 45.9% and 89.2% of other surfaces showed acceptable total bacterial and coliform count, respectively. Conclusion: The results showed that safe management of the kitchen, education of the staff and also improvement of the equipment used are necessary.

Keywords

  1. Marzano M, Balzaretti CM. Cook-serve method in
  2. mass catering establishments: Is it still appropriate
  3. to ensure a high level of microbiological quality and
  4. safety? Food control 2011; 22(12): 1844-50.
  5. Veiros M, Proença R, Santos M, Kent-Smith L, Rocha
  6. A. Food safety practices in a Portuguese canteen. Food
  7. Control 2009; 20(10): 936-41.
  8. Azevedo I, Albano H, Silva J, Teixeira P. Food safety
  9. in the domestic environment. Food Control 2014; 37:
  10. -6.
  11. Lund BM, O’Brien SJ. Microbiological safety of food
  12. in hospitals and other healthcare settings. Journal of
  13. Hospital Infection 2009; 73(2): 109-20.
  14. Buccheri C, Casuccio A, Giammanco S, Giammanco
  15. M, La Guardia M, Mammina C. Food safety in hospital:
  16. knowledge, attitudes and practices of nursing staff
  17. of two hospitals in Sicily, Italy. BMC health services
  18. research 2007; 7(1): 45.
  19. Konecka-Matyjek E, Maćkiw E, Krygier B, Tomczuk
  20. K, Stoś K, Jarosz M. National monitoring study on
  21. microbial contamination of food-contact surfaces in
  22. hospital kitchens in Poland. Annals of agricultural and
  23. environmental medicine: AAEM 2012; 19(3): 457-63.
  24. Lahou E, Jacxsens L, Daelman J, Van Landeghem F,
  25. Uyttendaele M. Microbiological performance of a food
  26. safety management system in a food service operation.
  27. Journal of Food Protection 2012; 75(4): 706-16.
  28. Polong T, Tombe B, Begani RK. Effectiveness of
  29. cleaning and sanitation of food contact surfaces in
  30. the PNG fish canning industry. DWU Research Journal
  31. ; 16: 68-81.
  32. ISO 18593:2004. Microbiology of food and animal
  33. feeding stuffs—horizontal methods for sampling
  34. techniques from surfaces using contact plates and
  35. swabs. 2004.
  36. ISO 4833-1:2013. Microbiology of the food chain
  37. —Horizontal method for the enumeration of
  38. microorganisms—Part 1:Colony count at 30 °C by
  39. the pour plate technique. 2013.
  40. BS ISO 4832:2006. Microbiology of food and animal
  41. feeding stuffs Horizontal method for the enumeration
  42. of coliform Colony count technique.2006.
  43. Domenech-Sanchez A, Laso E, Perez MJ, Berrocal
  44. CI. Microbiological levels of randomly selected food
  45. contact surfaces in hotels located in Spain during
  46. –2009. Food borne pathogens and disease 2011;
  47. (9): 1025-9.
  48. Legnani P, Leoni E, Berveglieri M, Mirolo G, Alvaro
  49. N. Hygienic control of mass catering establishments,
  50. microbiological monitoring of food and equipment.
  51. Food Control 2004; 15(3): 205-11.
  52. Di Ciccio P, Vergara A, Festino A, Paludi D, Zanardi E,
  53. Ghidini S, et al. Biofilm formation by Staphylococcus
  54. aureus on food contact surfaces:Relationship with
  55. temperature and cell surface hydrophobicity. Food
  56. Control 2015; 50: 930-6.
  57. Balzaretti CM, Marzano MA. Prevention of travelrelated
  58. foodborne diseases: Microbiological risk
  59. assessment of food handlers and ready-to-eat foods
  60. in northern Italy airport restaurants. Food control 2013;
  61. (1): 202-7.
  62. Saad M, See TP, Abdullah MFF, Nor NM. Use of Rapid
  63. Microbial Kits for Regular Monitoring of Food-contact
  64. Surfaces towards Hygiene Practices. Procedia-Social
  65. and Behavioral Sciences 2013; 105: 273-83.
  66. Garayoa R, Diez-Leturia M, Bes-Rastrollo M, Garcia-
  67. Jalon I, Vitas AI. Catering services and HACCP:
  68. Temperature assessment and surface hygiene control
  69. before and after audits and a specific training session.
  70. Food Control 2014; 43: 193-8.
  71. Soares K, Garcia-Diez J, Esteves A, Oliveira I, Saraiva
  72. C. Evaluation of food safety training on hygienic
  73. conditions in food establishments. Food Control 2013;
  74. (2): 613-18.
  75. Tofighi Sh, Hamouzadeh P, Sadeghifar J, Raad Abadi
  76. M, Roshani M, Salimi M, et al. The Compliance
  77. Status of HACCP Implementation Requirements in
  78. Nutrition Departments of the Selected Hospitals of
  79. Tehran University of Medical Sciences. Jundishapur
  80. Journal of Health Sciences 2012: 4(3): 15-23.
  81. Baniardalan M, Sabzghabaee AM, Jalali M, Badri Sh .
  82. Bacterial Safety of Commercial and Handmade Enteral
  83. Feeds in an Iranian Teaching Hospital. International
  84. Journal of Preventive Medicine 2014: 5(5): 604-10.