Antimicrobial Resistance Pattern in Escherichia coli Isolates Obtained from a Specialized Women and Children Hospital in Shiraz, Iran: A Prevalence Study

Mahtab Hadadi, Yalda Malekzadegan, Hamid Heidari, Hadi Sedigh Ebrahim-Saraie, Mohammad Motamedifar

Abstract


Abstract
Background: Escherichia coli, known as a clinically significant
bacteria, can cause a wide range of infections, including urinary
tract infections (UTIs), blood stream infections (BSIs), and
can frequently be isolated from various clinical specimens.
Evaluation of antimicrobial resistant pattern is a necessary
action, especially about such bacteria which are frequent and
life threatening. The aim of this study was to determine the
frequency and antimicrobial resistance pattern of E. coli isolates
obtained from various clinical specimens.
Methods: This retrospective study was performed within a seven
month period from January 2015 to August 2015 at a specialized
women and children hospital in Shiraz, Iran. E. coli isolates were
obtained from various clinical specimens and identified using
standard microbiological procedure. Antimicrobial susceptibility
patterns were determined using disk diffusion method in
accordance with CLSI recommendation.
Results: Of the total 130 positive cultures, the majority of E.
coli isolates were obtained from urine (96=73.8%) and blood
(11=8.5%) specimens. Overall, gentamicin (70.8%) was the
effective antibiotic for the tested E. coli isolates. E. coli isolates
obtained from urine specimens showed the highest resistance
rates against ampicillin (84.4%) and nalidixic acid (61.5%);
while they showed the most sensitivity to gentamicin (79.2%),
nitrofurantoin (70.8%) and ciprofloxacin (66.7%). Moreover,
the highest antibiotic resistance rates belonged to the isolates
recovered from endotracheal tube (ETT).
Conclusion: The results showed that gentamicin was the most
effective antibiotic against E. coli infections. However, in
addition to the gentamicin, we can recommend nitrofurantoin
and ciprofloxacin as the other effective agents for UTIs.


Keywords


Escherichia coli, Women, Children, Nosocomial infections, Antibiotic susceptibility

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