Narjes Moezi; Kourosh Azizi; Reza Sadeghi; Saideh Yousefi; Mozaffar Vahedi; Saeed Shahabi; Azim Paksa
Abstract
Background: Mosquitoes transmit many diseases to humans, including malaria, dengue, yellow fever, chikungunya, and Zika. Controlling mosquitoes with endosymbiont bacterium Wolbachia is a new approach in this field. This study aimed to determine the Wolbachia infection of two mosquito species, Aedes caspius ...
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Background: Mosquitoes transmit many diseases to humans, including malaria, dengue, yellow fever, chikungunya, and Zika. Controlling mosquitoes with endosymbiont bacterium Wolbachia is a new approach in this field. This study aimed to determine the Wolbachia infection of two mosquito species, Aedes caspius and Culex pipiens, in the city of Shiraz, southern Iran.Methods: Samples of Ae. caspius and Cx. pipiens were collected from four localities in Shiraz City, Fars Province. The samples were identified using the morphological identification keys. Collected samples were screened for Wolbachia infection using a PCR assay targeting the Wolbachia surface protein (wsp) gene.Results: Eight species from four genera were collected in this study; the most caught species was Cx. Pipiens, and the lowest abundant species was An. hyrcanus. From 110 adult Cx. pipiens screened using the wsp primer, 75 (68%) samples were infected with Wolbachia. The Wolbachia sequences in Cx. pipiens were like Wolbachia strains belonging to supergroups B. There was no Wolbachia infection in 204 Ae. caspius investigated samples.Conclusion: Our study revealed the presence of the supergroup B Wolbachia strain in Cx. pipiens samples. The present study did not detect any Wolbachia infection in Ae. caspius; however, it remains plausible to introduce Wolbachia populations into Wolbachia-free populations of this species. Such an introduction holds promise as a viable tool for vector control and mitigating the transmission of arboviral diseases such as West Nile virus and Chikungunya through cytoplasmic incompatibility.
Kourosh Azizi; Hedayat Dorzaban; Aboozar Soltani; Hamzeh Alipour; Seyed Aghil Jaberhashemi; Mostafa Salehi-Vaziri; Tahereh Mohammadi; Zahra Fereydouni; Azim 0000-0002-4167-5843 Paksa
Abstract
Background: Aedes mosquitoes transmit important arboviral diseases such as dengue to humans. This study was conducted to determine dengue virus infection in Aedes mosquitoes, emphasizing Aedes aegypti by Reverse Transcriptase-Polymerase Chain Reaction Assay from different regions in Southern Iran.Methods: ...
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Background: Aedes mosquitoes transmit important arboviral diseases such as dengue to humans. This study was conducted to determine dengue virus infection in Aedes mosquitoes, emphasizing Aedes aegypti by Reverse Transcriptase-Polymerase Chain Reaction Assay from different regions in Southern Iran.Methods: Aedes samples were collected by standard methods from different habitats of Hormozgan province, Southern Iran, in 2016-2017, and identified by morphological characteristics. In this study, TissueLyserII was used to homogenize the collected mosquitoes. In addition, the RT-PCR technique was used to identify dengue virus RNA.Results: Overall, 1351 larval and adult Aedes mosquitoes were collected from five sites in Hormozgan Province, including 452 adults and 899 larvae. Five species from Aedes genera were collected (Ae. aegypti, Ae. vittatus, Ae. caballus, Ae. caspius, Ae. vexans). The investigations of dengue virus infection in Aedes mosquitoes showed no dengue virus infection in this species.Conclusion: This study provides important information about Aedes mosquitoes. Vector control strategies must be emphasized and prioritized. Such actions prevent the establishment of Aedes mosquitoes and the spread of arboviral diseases in new areas. In addition, early detection of arboviruses in vectors and entomological monitoring can enhance the control measures for arbovirus diseases.
