Document Type: Original Article


1 Department of veterinary, Beyza branch,Islamic Azad University, Beyza, Iran

2 Student research committee, Shiraz University of Medical Sciences, Shiraz, Iran


The presence of antibiotic residues in milk and other livestock by products is a health hazard that can endanger the public health. Antibiotics are widely used in animal husbandry to treat bacterial infections. In this industry, antimicrobial drugs are being used for decades; as a result, continuous administration of antibiotics to livestock had led to contamination of industrialized dairy farms.
Background: This research was conducted to evaluate antibiotic residues in raw milk samples in Sepidan, using ECLIPS 50 kit and TWINE SENSOR kit.
Methods: In this cross-sectional study, one hundred raw cow milk samples were randomly collected from different farms and milk factories in Sepidan and Beyza townships from winter 2017 to spring 2018. The ECLIPS 50 and TWINE SENSOR kits were used to monitor antibiotic residues in milk samples. The data were analyzed employing Chi-‌square test, using SPSS software version 20. The significance level was considered p<0.05.
Results: In total, 100 raw milk samples were collected, of which 60 (60%) were from Beyza and 40 (40%) from Sepidan. A total of 95 samples (95%) were antibiotic-free and 5 (5%) contained antibiotic residual. 5 samples (5%) of ECLIPS 50 kit, 5 samples (5%) of TWINE SENSOR kit were shown to be positive, using both kits.
Conclusion: There was no difference between ECLIPS 50 kit and TWINE SENSOR kits in detecting antibiotics residue in raw milk samples. The positive samples in the two sets of kits were identical. Furthermore, there was no significant difference between the two types of kits