Document Type : Original Articles
Authors
- Kourosh Azizi
- Mohammad Djaefar Moemenbellah-Fard
- Mousa Khosravani-Shiri
- Mohammad Reza Fakoorziba
- Aboozar Soltani
Department of Medical Entomology, Research Center for Health Sciences, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract
Background: Human malaria remains a major malady in Eastern Iran. Vector control interventions such as indoor residual spraying are used to fight with the disease. This study was undertaken to determine the lethal and residual effects of three different pyrethroid insecticides on adult mosquitoes of Anopheles stephensi on different surfaces in Iran, as part of a national program to monitor insecticide resistance in endemic areas. Methods: Two main endemic foci were selected as collection sites. Wild adult females of An. stephensi (mysoriensis strain) from the first focus were subjected to standard susceptibility tests, using lambdacyhalothrin, deltamethrin and cyfluthrin insecticides within holding tubes according to the method proposed by WHO. In Kazerun, the residual effects of these compounds were examined by conical bioassay tests of An. stephensi (type strain) on plaster and cement walls. Data were analyzed using Mann-Whitney test to determine the differences in susceptibility and residual effects of An. stephensi mosquitoes to these insecticides. Results: The susceptibility of females of An. stephensi to three concentrations of lambdacyhalothrin, deltamethrin and cyfluthrin insecticides culminated in full scale mortality at the highest diagnostic dose. The maximal residual time of these three insecticides on plaster and cement walls was estimated to be about three months. There was no significant difference in the mortalities of An. stephensi on different sprayed surfaces (P=0.653). Conclusion: All field-collected An. stephensi populations exhibit gross susceptibility to all diagnostic doses of the three evaluated insecticides. In endemic areas, lambdacyhalothrin reveals a slightly longer residual activity than the other two insecticides.
Keywords
- Moemenbellah-Fard MD, Saleh V, Banafshi O,
- Dabaghmanesh T. Malaria elimination trend from a
- hypo-endemic unstable active focus in southern Iran:
- predisposing climatic factors. Pathog Glob Health.
- ;106(6):358-65.
- Glick JI. Illustrated key to the female Anopheles of
- southwestern Asia and Egypt (Diptera: Culicidae).
- Mosq Systemat. 1992;24:125-53.
- Alipour H, Ladonni H, Abaie MR, Moemenbellah-
- Fard MD, Fakoorziba MR. Laboratory efficacy tests
- of pyrethroid-treated bed nets on the malaria vector
- mosquito, Anopheles stephensi, in a baited excitorepellency
- chamber. Pak J Biol Sci. 2006;9(10):1877-83.
- Moemenbellah-Fard MD (editor) Malaria vector control
- and personal protection: including indoor residual
- spraying. Global Malaria Program, World Health
- Organization. 2008; 110 p.
- Rafinejad J, Vatandoost H, Nikpoor F, Abai MR,
- Shaeghi M, Duchen S, et al. Effect of washing on the
- bio-efficacy of insecticide-treated nets (ITNs) and
- long-lasting insecticidal nets (LLINs) against main
- malaria vector Anopheles stephensi by three bioassay
- methods. J Vector Dis. 2008;45(2):143-50.
- Ruzo LO, Casida JE. Metabolism and toxicology of
- pyrethroids with dihalovinyl substituents. Environ
- Health Perspect. 1977;21:285-92.
- Raeisi A, Abai MR, Akbarzadeh K, Nateghpour M,
- Sartipi M, Hassanzehi A, et al. Residual effects of
- deltamethrin WG 25% as a new formulation on different
- surfaces against Anopheles stephensi, in south eastern
- Iran. J Arthropod Dis. 2010;4(1):60-5.
- WHO. Test procedure for insecticide resistance
- monitoring in malaria vectors, bio-efficacy and
- persistence of insecticides on treated surfaces. WHO/
- CDS/CPC/MAL/98.12. World Health Organization,
- Geneva, 1998.
- Soltani A, Vatandoost H, Oshaghi MA, Enayati AA,
- Raeisi A, Eshraghian MR, et al. Baseline susceptibility
- of different geographical strains of Anopheles stephensi
- (Diptera: Culicidae) to temephos in malarious areas of
- Iran. J Arthropod Dis. 2013;7(1):56-65.
- Hanafi-Bojd AA, Vatandoost H, Oshaghi MA,
- Haghdoost AA, Shahi M, Sedaghat MM, et al.
- Entomological and epidemiological attributes for
- malaria transmission and implementation of vector
- control in southern Iran. Acta Trop. 2012;121(2):85-92.
- Vatandoost H, Hanafi-Bojd AA. Indication of
- pyrethroid resistance in the main malaria vector
- Anopheles stephensi from Iran. Asian Pac J Trop Med.
- ;5(9):722-6.
- Moemenbellah-Fard MD, Benafshi O, Rafinejad J,
- Ashraf H. Tick-borne relapsing fever in a new highland
- endemic focus of western Iran. Ann Trop Med Parasitol.
- ;103(6):529-37.
- Fakoorziba MR, Baseri A, Eghbal F, Rezaee S, Azizi
- K, Moemenbellah-Fard MD. Post-earthquake outbreak
- of cutaneous leishmaniasis in a rural region of southern
- Iran. Ann Trop Med Parasitol. 2011;105(3):217-24.
- Fakoorziba MR, Eghbal F, Hassanzadeh J,
- Moemenbellah-Fard MD. Cockroaches (Periplaneta
- americana and Blattella germanica) as potential
- vectors of the pathogenic bacteria found in nosocomial
- infections. Ann Trop Med Parasitol. 2010;104(6):521-8.
- Azizi K, Fakoorziba MR, Jalali M, Moemenbellah-Fard
- MD. First molecular detection of Leishmania major
- within naturally infected Phlebotomus salehi from a
- zoonotic cutaneous leishmaniasis focus in southern
- Iran. Trop Biomed. 2012;29(1):1-8.
- Abtahi M, Shayeghi M, Khoobdel M, Vatandoost H,
- Abaei MR, Akbarzadeh K. Persistence and residue
- activity of deltamethrin on indoor residual spraying
- surfaces against malaria vectors in southeastern Iran.
- Asian Pac J Trop Biomed. 2011;S271-S275.
- Abbott WS. A method of comparing the effectiveness
- of an insecticide. J Econ Entomol. 1925;18:265-7.
- Azizi K, Soltani A, Poodat A, Khodadadi M, Yaran
- M, Hasanvand B. Susceptibility of Anopheles stephensi
- against five current chemical insecticides. J Hormozgan
- Univ Med Sci. 2010;14(4):305-11.
- Vatandoost H, Abai MR, Abbasi M, Shaeghi M,
- Abtahi M, Rafie F. Designing of a laboratory model
- for evaluation of the residual effects of deltamethrin
- (K-othrine WP 5%) on different surfaces against
- malaria vector, Anopheles stephensi (Diptera:
- Culicidae). J Vector Dis. 2009;46(4):261-7.
- Ladonni H, Motabar M, Iranpour M. Residual effect
- of lambdacyhalothrin (Icon 10% WP) on different
- surfaces in south of Iran. Iranian J Publ Health.
- ;23(1-4):21-32.